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mouse anti vamp 2  (Synaptic Systems)


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    Synaptic Systems mouse anti vamp 2
    Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with <t>VAMP-2</t> ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).
    Mouse Anti Vamp 2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-vamp-2/104+008/pmc10832297-213-128-131
    Average 94 stars, based on 70 article reviews
    mouse anti vamp 2 - by Bioz Stars, 2026-10
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    Images

    1) Product Images from "Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells"

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells

    Journal: Molecular & Cellular Proteomics : MCP

    doi: 10.1016/j.mcpro.2023.100704

    Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with VAMP-2 ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).
    Figure Legend Snippet: Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with VAMP-2 ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).

    Techniques Used: Knock-Out, Injection, Expressing, Activation Assay, Labeling, Staining, Patch Clamp, Transmission Assay, Membrane

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    Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with <t>VAMP-2</t> ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).
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    Image Search Results


    Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with VAMP-2 ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).

    Journal: Molecular & Cellular Proteomics : MCP

    Article Title: Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells

    doi: 10.1016/j.mcpro.2023.100704

    Figure Lengend Snippet: Conditional knockout of Munc18 -1 indicates that it is not required for normal function of mouse auditory inner hair cells . A , immunolocalization of Munc18-1 ( green ) in a Munc18-1 fl/fl Math1-creER + conditional knockout mouse that has not been injected with tamoxifen (TAX), resulting in the normal expression of Munc18-1. Co-localization with VAMP-2 ( magenta ) indicates the expression of Munc18-1 in efferent synapses. B , injection of tamoxifen leads to activation of creER and conditional knockout of Munc18 -1 in IHCs. The immunolocalization pattern of Munc18-1 ( green ) is not impaired, indicating that the slight labeling of IHCs results from unspecific background staining (scale bars: 10 μm). C , auditory brainstem response (ABR) evoked by 80 dB clicks in tamoxifen-injected (red, N = 9 mice) and uninjected ( black , N = 5 mice) Munc18-1 fl/fl Math1-creER + mice. D , ABR audiograms from tamoxifen-injected ( red , N = 9) and uninjected ( black , N = 5) Munc18-1 fl/fl Math1-creER + mice. Thresholds measured from individual animals are displayed as crosses and are slightly offset laterally for better visibility. While recordings from one litter of injected animals ( red crosses , N = 5) indicated normal hearing thresholds as compared to uninjected animals ( black crosses , N = 5), recordings from a second litter of injected animals ( light red crosses , N = 4) indicated apparently elevated hearing thresholds. E , patch-clamp recording in perforated patch configuration from IHCs of tamoxifen-injected Munc18-1 fl/fl Math1-creER + mice ( red , n = 8 IHCs from N = 5 mice) indicates normal synaptic transmission from IHCs, since exocytic membrane capacitance increments (ΔC m ) and calcium currents (I Ca ) recorded in response to depolarizing pulses of varying duration are comparable to data acquired in B6 wildtype mice ( black , n = 10 IHCs from N = 5 mice).

    Article Snippet: The following primary antibodies were used: rabbit anti-VAMP-7 (232003, Synaptic Systems), rabbit anti-syntaxin-6 (110062, Synaptic Systems), rabbit anti-syntaxin-7 (110072, Synaptic Systems), rabbit anti-syntaxin-8 (110083, Synaptic Systems), rabbit anti-syntaxin-12/13 (110133, Synaptic Systems), rabbit anti-syntaxin-16 (110162, Synaptic Systems), rabbit anti-SCAMP1 (PA1-739, Thermo Fisher Scientific), mouse anti-V-ATPase (149011, Synaptic Systems), mouse anti-SV2B (119111, Synaptic Systems), rabbit anti-Vti1A (165002, Synaptic Systems), rabbit anti-PKC alpha [Y124] (ab32376, Abcam), rabbit anti-YKT6 (ab236583, Abcam), rabbit anti-VAP-A (249002, Synaptic Systems), mouse anti-VCP (MA3-004, Thermo Fisher Scientific), mouse anti-otoferlin [13A9] (ab53233, Abcam), rabbit anti-otoferlin (178003, Synaptic Systems), rabbit anti-VGluT3 (135203, Synaptic Systems), guinea pig anti-VGluT3 (135204, Synaptic Systems), mouse anti-synapsin-1 (106001, Synaptic Systems), goat IgG anti-CtBP2 [E−16] (sc-5967, Santa Cruz Biotechnology), rabbit anti-Munc18-1 (116002, Synaptic Systems), rabbit anti-Munc18-2 (116102, Synaptic Systems), rabbit anti-Munc18-3 (116202, Synaptic Systems), and mouse anti-VAMP-2 (104211, Synaptic Systems).

    Techniques: Knock-Out, Injection, Expressing, Activation Assay, Labeling, Staining, Patch Clamp, Transmission Assay, Membrane